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Assay of trimethoprim in plasma and urine by HPLC using electrochemical detection
Summary
Type:
HPLC
Application #:
103160
Catalog#:
720002.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x125mm
Detection:
UV, 280 or electrochemical detection at 1200 mV
Conditions:
eluent: 0.07M KH2PO4 pH 4.75 - methanol (3:1)
flow rate 1.5ml/min
pressure 200bar
Authors:
Nordholm L
Source:
J Chromatogr 233 (1982) 427 - 431
Details
Legend: Fig. 2. Electrochemical detection at 1200mV (top) and UV detection at 280nm (bottom) of A) blank plasma extract, B) plasma extract from a 67-day-old pig 7h after intravenous injection of 5mg/kg TMP.
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